The method of work in two phases:
The first phase of the work of the Cross Matching:
1. Attend one tube One Tube per unit (bag) of blood Blood Unit is required according to the needs of the patient.
2. Added to the tube two drops 2 drops of patient serum Patient Serum
3. Added to the tube two drops 2 drops of blood (bag) donatedDilution of Cells (blood) of donor4. Add two drops 2 drops of Bovine Albumin.
The second phase of the work of the Patient Control
1. Attend one tube One Tube for Control Control for each patientsample requests Cross Matching compatibility testing, regardless of the number of bags required to donate to him.
2. Added to the tube two drops 2 drops of patient serum Patient Serum
3. Added to the tube two drops 2 drops of patient serum Patient Cells
4. Add two drops 2 drops of Bovine Albumin.
Point in common:
1. Tube is placed the Cross Matching (first phase) and the tubePatient Control (Phase II) in Incubator at 37 ° C for 30 minutes.
2. Washing Washing tubes (Cross Matching Tube and Patient Control Tube) three times PAL Normal Saline in a manual orautomatic.
3. Added to the tubes two drops 2 drops of Coombs Serum orso-called Anti Human globulin (AHG).
4. Put your centrifuge tubes Centrifuge for 10-15 seconds in order to mix Mix well.
5. Examines the tubes under the microscope, if there is noclotting NonAgglutination are Compatible, and if clotting wasAgglutination Incompatible.
• If coagulation Agglutination under the microscope in a test tubeof the Cross Matching This indicates that there is no consensusbetween the patient and the donor bag, in this case is to change the bag last donor donated a bag and hold the same test of the above
• If you repeat a mismatch between the sample and the patient'sfuture, several bags of blood donors testing is conducted in a manner Cross Matching Warm Technique (technical warm) andby conducting each test of the Cross Matching above the temperature of 37 ° C.
